Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes
15. Juni 2014 Rita

My latest publication is a review about current state-of-the-art methodologies of membrane protein synthesis in cell-free systems. I focused on bio-mimetic supports and accentuated the advantages of biological membranes in cell-free systems.

It is published with open access, so I hope you have fun while reading it.

Abstract

When taking up the gauntlet of studying membrane protein functionality, scientists are provided with a plethora of advantages, which can be exploited for the synthesis of these difficult-to-express proteins by utilizing cell-free protein synthesis systems. Due to their hydrophobicity, membrane proteins have exceptional demands regarding their environment to ensure correct functionality. Thus, the challenge is to find the appropriate hydrophobic support that facilitates proper membrane protein folding. So far, various modes of membrane protein synthesis have been presented. Here, we summarize current state-of-the-art methodologies of membrane protein synthesis in biomimetic-supported systems. The correct folding and functionality of membrane proteins depend in many cases on their integration into a lipid bilayer and subsequent posttranslational modification. We highlight cell-free systems utilizing the advantages of biological membranes.

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Rita
Fraunhofer-Institut für Zelltherapie und Immunologie IZI
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